Application

Research Sub CategoryIntegrins

Research CategoryCell Structure

This Anti-CD49f Antibody, clone 4F10 is validated for use in FC, IP, IH for the detection of CD49f.

Immunohistological studies on frozen sections

Immunoprecipitation of the laminin receptor

Flow Cytometry

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Cell line SW/222 (Human colorectal carcinoma)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MAB2254

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

The monoclonal was purified by protein A affinity chromatography and is presented as a liquid in phosphate buffered saline containing 10mM sodium azide and 1 mg/mL bovine serum albumin.

Format: Purified

Specificity

This antibody is specific to the integrin alpha6 chain which is expressed on epithelial and endothelial basement membrane. The VLA alpha6 chain, which consists of a single disulphide linked 120 kDa subunit and a small doublet subunit of 25/30 kDa, is non covalently associated with the VLA beta-chain (CD29) in VLA-6 or alpha6/beta1 complex or with integrin beta4 chain (alpha6/beta4).

The antigen is also expressed on platelets, monocytes, thymocytes and T lymphocytes.

FUSION PARTNER: NS1 myeloma cell line

Storage and Stability

Store an 2-8°C for up to 12 months from date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C. Avoid repeated freeze-thaw cycles.